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The Golgi Apparatus

These images show the distribution of the Golgi apparatus within a cultured epithelial cell. The eukaryotic cell contains extensive internal membrane systems, such as the endoplasmic reticulum (ER) and the Golgi apparatus (GA). Once a protein is synthesized on a membrane-bound ribosome, it passes across the membrane of ER where the protein is processed by enzymes and folded into its correct three-dimensional conformation. Following the ER, the protein enters the Golgi apparatus where it is further modified by the addition of polysaccharide chains and packaged into vesicles. The ultimate fate of the protein is to be secreted out of the cell via exocytosis of the vesicles.

In plant cells, the Golgi apparatus is involved in the synthesis of polysaccharides used to build the cell wall surrounding the cell. While in animal cells, the Golgi apparatus is involved in the synthesis or modification of polysaccharide chains that are attached to proteins or lipids.

C. Golgi discovered the GA in 1898 using a silver impregnation technique for staining. Cells displayed here were processed using formaldehyde fixation and stained with rhodamine-conjugated antibodies to wheat germ agglutin to view the Golgi apparatus.

For further information on the Golgi apparatus, click the images below.

Golgi ApparatusGolgi Apparatus

Another subset of cells were processed using similar fixation, in addition they were also stained with sytox green, a DNA specific dye, to view the nucleus.

For further information on the Golgi apparatus,, click the images below.

Golgi ApparatusGolgi Apparatus


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