Stereology Workstation (SW)

Stereology Workstation

Using the Zeiss AxioImager A1 light microscope and the software provided, one may trace and analyze branching structures throughout single or serial sections. The SW will also plot and analyze the distribution of cells throughout single or serial sections. Measurements may be made while tracing, and the system includes all modern stereological probes for unbiased measurements (optical fractionator, optical and physical dissector, Cavalieri, cycloids, lines, nucleators, rotator).

A chart for immersion oil types can be found here.

ITG users may reserve time on the SW using the Stereology calendar.

Manufacturer:	Zeiss
Equipment Model:	Zeiss AxioImager A1 light microscope
Features:	Optronincs MicroFire Progressive Scan CCD -CCD Array: Large Format, ¾” Color CCD -Active Pixels: 1600 x 1200 -Pixel Size: 7.4um square -Fluoresence, transmitted brightfield, reflected brightfield & transmitted polarized light capabilities -Motorized stage and focus - StereoInvestigator software - Neurolucida software
Location:	B606E
Phone:	(217) 244-4473
Contact Person:	Jon Ekman Scott Robinson
Fees & Consumables:	See Microscopy Suite Fees
Related Equipment:	None
Related Forums:	StereoInvestigator: The Fuss About Using Stereology. Neurolucida and Stereoinvestigator: Two systems for Analysis of Morphology of Biological Structures.
Related Technical Reports:
File Format:	StereoInvestigator saves tracing data in either binary (by specifying a file extention of .DAT) or ASCII (file extention .ASC). Neurolucida saves the image files by default in MBF 8-bit binary files. Other formats supported are GIF, JPEG, TIF, TGA, PCX and PIW: use "Save As" to save the image file in one of those formats. Neuroexplorer (which is used for tracing analysis, including those done using Neurolucida and StereoInvestigator software) opens Neurolucida.DAT binary files, as well as ASC, ASCII, or NRX file formats. The data are saved as MicroBrightField.DAT files.