REFLECTED LIGHT MICROSCOPE
Zeiss Axiovert 135 inverted research microscope equipped with incident halogen lamp illumination and 100W Hg Lamp and is specifically suited for reflected light optics for visible spectrum CCD image capture and image feature measurement. The microscope is equipped with 10x, 20x and 40x long working distance objectives and is intended to fill the magnification gap between the dissecting microscope and high resolution imaging modalities such as SEM and Atomic Force Microscopy. The microscope is appropriate for viewing opaque specimens which can not be readily observed using conventional transmitted light microscopy. Images are captured via a Roper Scientific CoolSnap color CCD camera using Image Pro Plus software. Reflected Near IR images are captured via a base port mounted Flir Alpha NIR camera. Images may be captured IRVista software from Indigo. The scope is equipped with two optical fiber attached spectral detectors covering Visible 400-900 nm & NIR IR 1000 -1700 nm from Control Development, Inc. The scope can also be fitted with laser tweezers and microinjector for live cell imaging. Tables for the Zeiss Objectives can be found here. A chart for immersion oil types can be found here. ITG users may reserve time on the LM using the reflected light
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