Fluorescence Light Microscope (LM)

Axiovert 200M

The Zeiss Axiovert 200M inverted research-grade microscope is configured to perform fluorescence microscopy as well as differential-interference contrast (Nomarski) and phase-contrast microscopy. The addition of a stage heater, motorized stage, and shutters to minimize light exposure allows users of the facility to perform live cell microscopy. The entire system is driven using Zeiss Axiovision, a sophisticated software package which facilitates image collection. A particularly useful feature of this software is the ability to montage multiple fields of view, allowing composition of images of very large specimens. Images may be acquired using a Roper Scientific Cascade 512B EMCCD camera or a Zeiss AxioCam HRC color CCD camera. The system is also equipped with Zeiss Apotome structured illumination system to increase widefield fluorescence contrast and for optical sectioning.

Tables for the Zeiss Objectives can be found here.

A chart for immersion oil types can be found here.

ITG users may reserve time on the LM using the Fluorescence calendar.

Manufacturer:	Carl Zeiss, Inc.
Equipment Model:	Axiovert 200
Features:	- B/W EMCCD with 90% QE - High resolution color CCD - phase contrast high NA DIC - Zeiss Axiovision Software - Time-lapse - Z-stacking - Mark & Find - Tiling - Apotome structured illumination - stage heater - motorized stage - shutters to minimize light exposure
Location:	B606B
Phone:	in hallway: (217) 244-7221
Contact Person:	Jon Ekman Duohai Pan
Fees & Consumables:	See Microscopy Suite Fees
User Resourcecs :	General Information and Operating Procedures AxioVision LE Rel. 4.5 viewer software Filter sets
Related Equipment:	None
Related Forums:	The Internet Atlas of Histology. Automated Quantitation of Labeled Cells: The MCID System in Action. Using Optical Tweezers to Manipulate Cells. A Web Atlas of Cellular Structures using Light and Confocal Microscopy. An Introduction to the Light Microscope in the ITG.
Related Technical Reports:	Cell Biological Applications of Fluorescence Microscopy. A Web Atlas of Cellular Structures Using Light and Confocal Microscopy.
File Format:	MCID allows users to save images acquired with the software as either .tiff files or as MCID files, a proprietary format which is only recognized by the AIS or MCID software packages.