Leica SP2 Confocal And Multiphoton Microscope

Confocal microscopy permits one to optically section a fluorescent sample (such as a cell that has been stained with contrasting fluorescent dyes) with superior resolution by using a pinhole to reject light that originates outside of the chosen area. By collecting a series of such images through the depth of a sample, the user may assemble a highly accurate three-dimensional reconstruction of the entire sample. The Leica SP2 confocal microscope is equipped with a spectral head that employs a prism, moveable slits, mirrors, and computer control to permit the operator to choose which bands of light at specific wavelengths will be focused simultaneously onto each of three photomultiplier detectors. This system also allows emission spectra (with 5-nm resolution) to be collected from a diffraction-limited-size spot. In addition, the microscope is equipped for multiphoton imaging. A laser is used to produce short (femtosecond) pulses of extremely high intensity near infrared (relatively long wavelength) light. When this light is focused on the sample, a dye molecule can absorb two low energy (long wavelength) photons simultaneously. The dye molecule will then emit a single high energy (short wavelength) photon, which can be detected as visible light. The advantage of multiphoton microscopy is its ability to supply low energy photons, which are not absorbed by tissue, to excite fluorescently labeled cells or materials.

ITG users may reserve time on the Leica SP2 using the Leica SP2 #1 calendar.